ABSTRACT
Objective:To test the survival ofStaphylococcus aureus (S. aureus) inside lymphocyte that contributes to the pathogenesis of infection and possible anti-inflammatory and antioxidative effect of nanoconjugated vancomycin againstin vivo S. aureus infection in a dose and duration dependent manner.Methods:5×106CFU/mL vancomycin-sensitive S. aureus(VSSA) and vancomycin-resistiveS. aureus (VRSA) were challenged in Swiss male mice for3 days,5 days, 10 days and15days, respectively. Bacteremia and inflammatory parameters were observed to evaluate the duration for development ofVSSA andVRSA infection.100mg/kg bw/day and500 mg/kg bw/day nanoconjugated vancomycin were administrated toVSSA andVRSA infected group for5days. Bacteremia, inflammatory parameters and oxidative stress related parameters were tested to observe the effective dose of nanoconjugated vancomycin againstVSSAandVRSA infection. Nanoconjugated vancomycin was treated at a dose of100 mg/kg bw/day and500 mg/kg bw/day, respectively, toVSSA andVRSA infected group for successive5 days,10 days and 15 days. Bacteremia, inflammatory parameters and oxidative stress related parameters were observed to assess the effective duration of nanoconjugated vancomycin againstVSSAand VRSA infection.Results: The result revealed thatin vivoVSSA andVRSA infection developed after 5 days of challenge by elevating theNO generation in lymphocyte and serum inflammatory markers. Administration with nanoconjugated vancomycin toVSSA andVRSA infected group at a dose of100 mg/kg bw/day and500 mg/kg bw/day, respectively, for successive10 days eliminated bacterimia, decreasedNO generation in lymphocyte, serum inflammatory markers and increased antioxidant enzyme status.Conclusions:These findings suggest,in vivochallenge ofVSSA andVRSA for5 days can produce the highest degree of damage in lymphocyte which can be ameliorated by treatment with nanoconjugated vancomycin for10 successive days.
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Objective: To observe the biochemical characters and antibiotic susceptibility of isolated Staphylococcus aureus (S. auerus) strains against some conventional and traditional antibiotics.Methods:Bacterial culture was done in Mueller-Hinton broth at 37 ℃. Characters of these strains were determined by traditional biochemical tests such as hydrolysis test of gelatin, urea, galactose, starch and protein, and fermentation of lactose and sucrose. Antibiotic susceptibility were carried out by minimum inhibitory concentration test, minium bactericidal concentration test, disc agar diffusion test and brain heart infusion oxacillin screening agar. Results: From this study, it was observed that 100% S. aureus isolates showed positive results in gelatin, urea and galactose hydrolysis test, 50% isolates were positive in starch hydrolysis test, 35% in protein hydrolysis test, 100% isolates in lactose fermenting test, but no isolate was positive in sucrose fermenting test. Antibiotic susceptibility testing suggested that 20% of isolates were resistant to kanamycin and 46.67% were resistant to oxacillin. Conclusions: These findings show that all these isolates have gelatin, urea, galactose hydrolysis and lactose fermenting activity. 20% of these isolates were resistant to kanamycin and 46.67% were resistant to oxacillin. Thirty post operative pathogenic isolated S. aureus strains were used in this study.
ABSTRACT
Objective: To evaluate the potency of carboxymethyl chitosan-2, 2’ ethylenedioxy bis-ethylamine-folate (CMC-EDBE-FA) on tissue injury, antioxidant status and glutathione system in tissue mitochondria and serum against nicotine-induced oxidative stress in mice. Methods:CMC-EDBE-FA was prepared on basis of carboxymethyl chitosan tagged with folic acid by covalently linkage through 2, 2’ ethylenedioxy bis-ethylamine. Animals were divided into four groups, i.e., control, nicotine (1 mg/kg bw/day), CMC-EDBE-FA (1 mg/kg bw/day) and nicotine (1 mg/kg bw/day) and CMC-EDBE-FA (1 mg/kg bw/day) for 7 days. Levels of lipid peroxidation, oxidized glutathione level, antioxidant enzyme status and DNA damage were observed and compared. Results: The significantly increase of lipid peroxidation, oxidized glutathione levels and DNA damage was observed in nicotine treated group as compared with control group; those were significantly reduced in CMC-EDBE-FA supplemented group. Moreover, significantly reduced antioxidant status in nicotine treated group was effectively ameliorated by the supplementation of CMC-EDBE-FA. Only CMC-EDBE-FA treated groups showed no significant change as compared with control group; rather than it repairs the tissue damage of nicotine treated group. Conclusions: These findings suggest that CMC-EDBE-FA is non-toxic and ameliorates nicotine-induced toxicity.